Culture media Hektor, InVitrus, TurboDoma
Fortified culture media InVitrus VP-6, TurboDoma TP-6

Packaging
Hektor, InVitrus & TurboDoma media kits consist of 5 vials containing liquid solutions.
InVitrus VP-6 & TurboDoma TP-6 media kits consist of 6 vials containing liquid solutions.
Sodium hydrogen carbonate and L-glutamine have to be added separately.
 

Storage
Store the kit at -20ºC for optimal preservation.
 

Preparation
After thawing, shake the vials vigorously before use!
Time required for preparing the medium for sterile filtration: 10 minutes.

 

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1. Measure out 18 liters of double distilled or purified tissue culture water at ambient temperature
   in a stirred vessel. While stirring, add the solutions without heating the water and ensure full recovery of the material from the vials by rinsing them well.
    
2. Add solutions no. 1 and no. 2, then exactly 45.0 g sodium hydrogen carbonate and wait until complete dissolution.
   The recommended L-glutamine concentration is 4mM (11.68 g for 20 liters medium).
   Note that higher glutamine levels may lead to excess cytotoxic catabolite formation.
    
3. Hektor, InVitrus & TurboDoma media
   Add solutions no. 3, no. 4 and no. 5. Adjust end volume with water.
   Do not adjust pH (the media are buffered with MOPS)!

   InVitrus VP-6 & TurboDoma TP-6 media:
   Add solutions no. 3, no. 4, no. 5 and no. 6. Adjust both end volume and, if necessary, pH to 0.1-0.2 units below the desired value with gaseous carbon dioxide.  

4. Sterilize by filtration and store refrigerated at 4-6ºC in the dark.

 

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Warning
The filter sterilized medium without L-glutamine expires 9 months after preparation.
Do not store small volumes of culture media in large vessels and protect them from light!