Gravesano, July 2026
Focus on InVitroNutrition for hematopoietic cells
One Defined Medium, Multiple Immune Cell Fates
Ongoing research using physiological InVitroNutrition media has shown how a chemically defined culture environment can reveal cellular and metabolic features that may remain hidden in conventional media. Building on these results, the InVitroNutrition approach is now being extended to hematpoietic cell culture, where precise control of the extracellular environment is particularily important.
The hidden variable in cell culture
A major challenge in cell culture is achieving reliable proliferation while preserving control over cell identity and function.
Fetal bovine serum can support cell proliferation, but it also introduces an incompletely defined mixture of proteins, lipids, hormones, cytokines, growth factors, and extracellular particles. These components may vary between batches and can influence differentiation independently of the signals intentionally added by the researcher.
For hematopoietic stem and progenitor cells, this is particularly relevant. Small changes in the culture environment can redirect cell fate, generate mixed populations, or produce phenotypes that are difficult to reproduce and interpret.
A defined environment for controlled differentiation
InVitroNutrition media provide a chemically defined physiological foundation in which nutrients and basal components are controlled. Growth factors, cytokines, lipids, and other differentiation signals can then be freely at selected concentrations.
This separates cellular nutrition from experimental signaling. The medium supports cell growth, while the researcher defines the differentiation pathway.
Using specific cytokine strategies, hematopoietic stem and progenitor cells can be expanded and directed toward macrophages, dendritic cells, or other myeloid derivatives. We also observed that with the same basal media cells like macrophages can then be further polarized toward distinct functional states by modifying only the selected differentiation signals.
The same basal medium can therefore support multiple cell fates without introducing the uncontrolled background associated with serum-containing systems.
From one starting population to multiple immune phenotypes
A defined culture environment makes it possible to follow more precise differentiation paths. Instead of relying on a complex serum background, researchers can adjust individual components and evaluate how each signal affects proliferation, maturation, population composition, and phenotype.
It also facilitates protocol optimization, because individual components can be adjusted without changing the entire biological background of the culture.
This is especially valuable when generating polarized macrophages or dendritic cells, where subtle differences in cytokine exposure can lead to clearly different functional states.
The result is a more adaptable platform for studying immune-cell plasticity, inflammation, antigen presentation, lipid metabolism, and disease-specific immune responses.
Research in progress
Current studies are evaluating InVitroNutrition media for the proliferation and directed differentiation of hematopoietic stem and progenitor cells into macrophages, polarized macrophage phenotypes, dendritic cells, and other myeloid derivatives.
The macrophage and dendritic-cell datasets are currently under scientific review and cannot yet be published. These ongoing studies support the broader goal of the InVitroNutrition platform: to give researchers greater control over cell fate by replacing undefined culture conditions with a physiological and chemically defined environment.
One defined medium. Multiple immune cell fates. Greater control over every differentiation step.
Fetal bovine serum can support cell proliferation, but it also introduces an incompletely defined mixture of proteins, lipids, hormones, cytokines, growth factors, and extracellular particles. These components may vary between batches and can influence differentiation independently of the signals intentionally added by the researcher.
For hematopoietic stem and progenitor cells, this is particularly relevant. Small changes in the culture environment can redirect cell fate, generate mixed populations, or produce phenotypes that are difficult to reproduce and interpret.
A defined environment for controlled differentiation
InVitroNutrition media provide a chemically defined physiological foundation in which nutrients and basal components are controlled. Growth factors, cytokines, lipids, and other differentiation signals can then be freely at selected concentrations.
This separates cellular nutrition from experimental signaling. The medium supports cell growth, while the researcher defines the differentiation pathway.
Using specific cytokine strategies, hematopoietic stem and progenitor cells can be expanded and directed toward macrophages, dendritic cells, or other myeloid derivatives. We also observed that with the same basal media cells like macrophages can then be further polarized toward distinct functional states by modifying only the selected differentiation signals.
The same basal medium can therefore support multiple cell fates without introducing the uncontrolled background associated with serum-containing systems.
From one starting population to multiple immune phenotypes
A defined culture environment makes it possible to follow more precise differentiation paths. Instead of relying on a complex serum background, researchers can adjust individual components and evaluate how each signal affects proliferation, maturation, population composition, and phenotype.
It also facilitates protocol optimization, because individual components can be adjusted without changing the entire biological background of the culture.
This is especially valuable when generating polarized macrophages or dendritic cells, where subtle differences in cytokine exposure can lead to clearly different functional states.
The result is a more adaptable platform for studying immune-cell plasticity, inflammation, antigen presentation, lipid metabolism, and disease-specific immune responses.
Research in progress
Current studies are evaluating InVitroNutrition media for the proliferation and directed differentiation of hematopoietic stem and progenitor cells into macrophages, polarized macrophage phenotypes, dendritic cells, and other myeloid derivatives.
The macrophage and dendritic-cell datasets are currently under scientific review and cannot yet be published. These ongoing studies support the broader goal of the InVitroNutrition platform: to give researchers greater control over cell fate by replacing undefined culture conditions with a physiological and chemically defined environment.
One defined medium. Multiple immune cell fates. Greater control over every differentiation step.
It also facilitates protocol optimization, because individual components can be adjusted without changing the entire biological background of the culture.
This is especially valuable when generating polarized macrophages or dendritic cells, where subtle differences in cytokine exposure can lead to clearly different functional states.
The result is a more adaptable platform for studying immune-cell plasticity, inflammation, antigen presentation, lipid metabolism, and disease-specific immune responses.
Research in progress
Current studies are evaluating InVitroNutrition media for the proliferation and directed differentiation of hematopoietic stem and progenitor cells into macrophages, polarized macrophage phenotypes, dendritic cells, and other myeloid derivatives.
The macrophage and dendritic-cell datasets are currently under scientific review and cannot yet be published. These ongoing studies support the broader goal of the InVitroNutrition platform: to give researchers greater control over cell fate by replacing undefined culture conditions with a physiological and chemically defined environment.
One defined medium. Multiple immune cell fates. Greater control over every differentiation step.
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