Culture media ChoMaster HP-1, ChoMaster HP-5
Fortified culture medium ChoMaster HP-6

Packaging
ChoMaster HP-1 & ChoMaster HP-5 media kits consist of 5 vials containing liquid solutions.
The ChoMaster HP-6 medium kit consists of 6 vials containing liquid solutions.
Sodium hydrogen carbonate and L-glutamine have to be added separately.
 

Storage
Store the kit at -20ºC for optimal preservation.
 

Preparation
After thawing, shake the vials vigorously before use!
Time required for preparing the medium for sterile filtration: 10 minutes.

 

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1. Measure out 180 liters of double distilled or purified tissue culture water at ambient temperature
   in a stirred tank. While stirring, add the solutions without heating the water and ensure full recovery of the material from the bottles by rinsing them well.
    
2. Add solutions no. 1 and no. 2, then exactly 504 g sodium hydrogen carbonate and wait until complete dissolution.
   The amount of L-glutamine to be added to the medium depends on the type of CHO cell line used, i.e. CHO-K1, CHO-DUKXB11, or CHO-DG44. Please contact us to identify the most suitable L-glutamine concentration for your particular application.
    
3. ChoMaster HP-1, ChoMaster HP-5 media
   Add solutions no. 3, no. 4 and no. 5. Adjust both end volume and pH to 0.1-0.2 units below the optimal value of 7.2 with gaseous carbon dioxide.

   ChoMaster HP-6 medium
   Add solutions no. 3, no. 4, no. 5 and no. 6. Adjust both end volume and pH to 0.1-0.2 units below the optimal value of 7.2 with gaseous carbon dioxide.  

4. Sterilize by filtration and store refrigerated at 4-6ºC in the dark.

 

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Warning
The filter sterilized medium without L-glutamine expires 9 months after preparation.
Do not store small volumes of culture media in large vessels and protect them from light!